Revista de biología vectorial

Classical Microbiological Diagnostics Of Bacteraemia - Are The Negative Results Really Negative? What is Laboratory Result telling us About “Gold Standard”?

Tomasz Wojciech Zrodlowski

Standard blood cultures require at least 24-120 h to be reported as preliminary positive. The objective of this study was to match the equivalent of Gram staining and fluorescent in-situ hybridization (FISH) for bacteria in otherwise negative blood culture bottles. Ninety-six sets were arrested from patients with a analysis of sepsis. Six incomplete blood culture sets and eight blood cultures sets professed positive growth were refuse. We achieve Gram's Method and FISH on 82 sets taken from check septic patients: 82 negative aerobic blood cultures, 82 anaerobic blood cultures, and 82 blood samples, as well as 57 blood samples taken from active volunteers. Against eighty-two blood agreed analysed from the septic patients, Gram's Method visualized bacteria in 62.2% of blood samples, 35.4% of the negative aerobic bottles, and in 31.7% of the negative anaerobic bottles. Apply FISH; we detected bacteria in 75.6%, 56.1%, and 64.6% commonly. Among the blood samples from healthy volunteers, FISH detected bacteria in 64.9%, while Gram's Method detected bacteria in just 38.6%. The time needed to get the study results using Gram's Method was 1 h, for FISH 4 h, and for the culture method, considering the duration of growth, 5 days. Gram stain and FISH allow quick detection of bacteria within the blood taken directly from a patient. Discovery phagocytized bacteria, which were also catch among healthy individuals, confirms the hypothesis that blood micro biome exists.