Informes de microbiología médica

Evalvuation of efficacy of saclac globule formulation of indigenous bacteriophages against multidrug resistant bacterial pathogens in water microcosm

Sangeeta Ahiwale

Bacteriophages are the viruses of prokaryotes that can either instantly kill a bacterial cell or integrate their genome into the host chromosome. In this study, potent lytic phages were isolated from the natural resources. They were characterized and their infectivity abilities against specific pathogens were checked. Saclac globule formulations using efficient phages were prepared and their shelf-life was checked at 4ºC and 30 ºC. Efficacy of saclac globule monovalent formulations of indigenous φ SPB (Salmonella phage) and BVPaP-3 (Pseudomonas phage) were evaluated against Salmonella enterica serovar Paratyphi B and Pseudomonas aeruginosa respectively in the water microcosms prepared using river water and swimming pool water spiked independently with the mentioned respective pathogens at different environmental parameters,viz.,growth phase of the cell (log and stationary), incubation conditions (shaking and static) and varied MOI (multiplicity of infection) values (MOI˂1, MOI=1, MOI=50 and MOI=100). Saclac globules contain goat milk sugar that keeps the bacteria in a log phase of growth for long period of time. At different MOI values, there was a great difference in the inactivation rate of Salmonella enterica serovar paratyphI B and Pseudomonas aeruginosa with maximum inactivation rate at MOI=100.There was a significant difference (P˂ 0.05) in pathogen inactivation rate at shaking and static condition. The rate of inactivation of log phase cells were higher than the stationary phase cells at shaking as well as static conditions at MOI=50 and MOI=100.