Revista de técnicas inmunológicas y enfermedades infecciosas

Electrochemical Biosensor for the Detection of Viruses and Possibly Covid-19

Mohamed Amine Smaini, Raja Maallah, Imane Smaini, Salah Eddine El Qouatli, Rachida Najih and Abdelilah Chtaini

The bio electrochemical behavior of West Nile virus at mixed carbon-phosphate paste electrode modified with PeroxidaseSecondary Antibody Conjugate (PAS) is reported. A Cyclic Voltammetry (VC), Square Wave Voltammetry and Impedance Spectroscopic methods were investigated. The influence of variables such as the accumulation time, pH solution and PAS loading was tested by different electrochemical methods. The detection limit is 1.8.10-3 and the quantification limit is 7.2.10- 2. The bio electrode developed has shown high efficiency for the detection of antibodies to the viruses tested but also shows promising signs for the detection of covid-19 antibodies