Biología celular: investigación y terapia

Dicer and Drosha Expression Levels in Ovarian Cancer Tissues

Sanaz Razmkhah*, Saeed Ghorbian, Rahman Ghaffarzadekan Rabati, Naghmeh Mohassel

MicroRNA (Mirnas) play crucial roles in diverse cellular processes and associate with various Ovarian Cancers (OC). Dicer and Drosha are two major enzymes in the miRNA maturation process. Dicer and Drosha genes expression investigated OC and analyzed the impact of clinicopathological characteristics on their expression. We investigated the expression of Dicer and Drosha in invasive (n=50), matched adjacent marginal tissue (n=50) using real-time RT-PCR. The expression rate change in the Dicer and Drosha genes was assessed using the 2-ΔΔCT method. The statistical analysis of genes expression levels showed that Dicer and Drosha were up-regulated, significantly increased in tumors compared to marginal tissue. On the other hand, there was no correlation between the expression of Dicer and Drosha to age and tumor size. This article serves as an early laboratory diagnostic and non-invasive prognosis in OC patients. These genes may have a potential role in malignancy and increase tumor stage by influencing miRNA molecule level and serve as markers for disrupted miRNA. Additional research should investigate the
different disease stages.